Start a new ARIA project with calibrated data in ARIA's .list format:

ARIA's .list format looks like that:
p DIST 962 1 1.00 0.124E+05 - - - - 0.000E+00 0.790E+01 0.000E+00 - 0.000E+00 0.887E+01 0.000E+00
a 3 0.00 1.00 2.00 5.50 4.56 0.00 - - 2 2 
c 1.00 1.00 4.56 0.00 0.00 0.00 - 49 MET N 404.000 0.000 HN 405.000 0.000 - 47 SER N 44.000 0.000 HN 45.000 0.000
c 1.00 10.17 46.42 0.00 0.00 0.00 - 75 VAL N 386.000 0.000 HN 387.000 0.000 - 47 SER N 44.000 0.000 HN 45.000 0.000
Please read the description of the format in the manual. Just append the files if you have more than one file.

1. Edit the directory and file names:

Current ARIA program directory: 
Path of the new project:        
Run number:	                
SPECTRUM 1: Name:               
            .tbl file:		
            .ppm file:		

SPECTRUM 2: Name:               
            .tbl file:		
            .ppm file:		

SPECTRUM 3: Name:               
            .tbl file:		
            .ppm file:		

SPECTRUM 4: Name:               
            .tbl file:		
            .ppm file:		

SPECTRUM 5: Name:               
            .tbl file:		
            .ppm file:		
Sequence or PDB file:           
Dihedral angle restraints:      
Karplus restraints 1:           
Karplus restraints 2:           
Karplus restraints 3:           
Karplus restraints 4:           
Karplus restraints 5:           
Residual dipolar couplings 1:   
Residual dipolar couplings 2:   
Residual dipolar couplings 3:   
Residual dipolar couplings 4:   
Residual dipolar couplings 5:   
Hbonds restraints:              
Chemical shift index file:      
Segid of the protein:           


You have to specify absolute paths for all files and directories. Unused fields should be blank. You need to specify a sequence as a sequence or as a PDB file. The deltas for the chemical shifts in F1 and F2 have to be specified for every input spectrum. Be careful not to use pathnames with more than 80 characters (otherwise CNS will complain). If you don't have ambiguous data, leave the corresponding field blank.


2. Please save the updated parameters somewhere in a directory as new.html:





3. Type ARIA on the UNIX command line in that directory where you have saved your new.html.